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. 2017 Jun 8;7:245. doi: 10.3389/fcimb.2017.00245

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Copyright © 2017 Malhotra, Patidar, Boradia, Kumar, Nimbalkar, Kumar, Gani, Kaur, Garg, Raje and Raje.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Surface binding of Lf. (A,B) FACS analysis indicates specific binding of Lf-HF488 on the surface of M.tb H37Ra cells (A) Overlay histogram plot of one representative experiment indicating log fluorescence of Lf-HF488. (B) Bar graph depicting MFI ± SEM. (C–D) TEM images reveal the binding of Lf conjugated gold particles (indicated by arrow heads) and (E) Control cells treated with control Streptavidin gold particles, demonstrate no surface binding of gold particles. Scale bar 0.2 μM. Multiple fields were analyzed with each sample, representative images are shown.