Table 1.

Analysis of mutants by computational methods.

Mutations (predicted stability change ΔΔG)	mCSM	SDM	DUET	Conservation score
N142S	−1.216	−0.36	−1.223	Highly (8–9)
P295L	−0.994	0.67	−0.793	Lower (1–2)

ΔΔG in Kcal/mol, negative values indicate destabilization and positive values indicate stability of protein structure.

mCSM: Predicts the effect of mutations in proteins using graph-based signatures.

SDM: Site Directed Mutator is a statistical potential energy function method.

DUET: Protein Stability Change upon Mutation methods.