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Establishment of L1CAM-depleted cells.
L1CAM shRNA, or a control shRNA, were transduced using lentiviral delivery into EGI-1 cells; a stable L1CAM-knockdown cell line was subsequently established. The expression level of L1CAM was determined by RT-PCR (A), Western blot analysis (B) and flow cytometry (C). Results are representative of three independent experiments.
