Figure 5.

Freeze‐thaw experiments and incubation experiments confirmed the stability of hsa_circ_0014717 in gastric juice. (A) Freeze‐thaw experiments. Four gastric juice samples were randomly selected and then equally divided to four parts. After 0, 2, 4, and 8 cycles of freeze‐thaw, the hsa_circ_0014717 levels were detected by qRT‐PCR. Three independent experiments were performed. P > 0.05. (B) Incubation experiments. Four gastric juice samples were first randomly selected and then equally divided to four parts. Then, they were stored at 4°C. After 0, 2, 4, and 8 h incubation, the hsa_circ_0014717 levels were detected by qRT‐PCR. Three independent experiments were performed. P > 0.05.