Figure 4.

Tie1 expression positively correlates with cancer stem properties in vivo. (A,B) Gene expression analysis of Lgr5, a cancer stem cell marker (A) and CK20, a typical differentiation marker (B) of intestinal tissue. Sorted Tie1‐positive and ‐negative cells recovered from transplanted tumors were analyzed by quantitative RT‐PCR. (C) Gene expression analysis of Tie1 in recovered HT29‐EGFP cells after continuous 5‐FU treatment. Quantitative RT‐PCR analysis was performed on transplanted HT29‐EGFP cells after selection pressure by 5‐FU. Tumor cells were sorted using EGFP signals. Each quantitative RT‐PCR experiment was performed at least three times. Expression levels were normalized to GAPDH, an internal control transcript. Bar represents SD.