Table 1.
A summary of mutant sources of SNP markers and KASP genotyping assays developed and validated in various genetic materials for different seed composition traits used in the present study.
| Trait | Marker | Assay ID | Gene | Position | Effect on trait | Donor checks | Reference |
|---|---|---|---|---|---|---|---|
| Oleic acid | FAD2-1A_17D | MU-HO-1 | Glyma10g42470 | S117N | Improves oil shelf life | 17 D | [46] |
| FAD2-1A_A_Del | MU-HO-2 | ∗A544 | PI603452 | [47] | |||
| FAD2-1B_327 | MU-HO-3 | Glyma20G24530 | P137R | PI283327 | [32] | ||
| FAD2-1B_I143T | MU-HO-4 | I143T | PI578451 | [32] | |||
|
| |||||||
| Linolenic acid | FAD3A_Del | MU-LL-1 | Glyma14g37350 | 6.4 kb del. | Oxidative and flavor instability | IA3024 | [31] |
| FAD3A_Splice | MU-LL-2 | G810A | CX1512-44; SB-01 | [48] | |||
| FAD3B_Splice | MU-LL-3 | Glyma02g39230 | G462A | IA3024 | [49] | ||
| FAD3C_G128E | MU-LL-4 | Glyma18g06950 | G128E | CX1512-44; SB-01 | [48] | ||
| FAD3C_H304Y | MU-LL-5 | H304Y | A29, IA3024 | [49] | |||
|
| |||||||
| Kunitz trypsin inhibitor | KTI | MU-KTI-1 | Glyma08g45530 | Q120∗ | Antinutritional | PI 542044 | [9] |
|
| |||||||
| Stachyose synthase | STS | MU-STS-1 | Glyma19g40550 | 33 bp del. | Antinutritional | SP6A-209 | [17] |
|
| |||||||
| Raffinose synthase | RS2_W | MU-RS2-1 | Glyma06g18890 | ∗TGG991 | Antinutritional | PI 200508 | [50] |
| RS2_397 | MU-RS2-2 | T107I | Antinutritional | 397 | [46] | ||
|
| |||||||
| Lipoxygenase | Lox-1 | MU-Lox-1 | Glyma13g42320 | 74 bp del. | Beany flavor | PI 408251 | [7] |
| Lox-2 | MU-Lox-2 | Glyma13g42310 | H532Q | Beany flavor | Jinpumkong-2 | [8] | |
| Lox3_G_100 | MU-Lox-3 | Glyma15g03030 | ∗G100 | Beany flavor | PI 205085; PI 417458 |
[7] | |
∗: deletion.