Table 1.

Thermal cycling profiles used in the Flavivirus RT-qPCR.

Standard (4.5 h, 25 μL)	Fast (2.5 h, 25 μL or 10 μL)
(1) 50°C for 30 min (2) 95°C for 15 min (3) 95°C for 15 s (4) 55°C for 25 s + plate read (5) 72°C for 25 s + plate read (6) 80°C for 1 s + plate read (7) GOTO (3); 44 more times (8) 95°C for 1 min (9) Melting curve, 68 to 88°C, increment 0.1°C, 1 s + plate read (10) 4°C forever (optional) End	(1) 50°C for 30 min (2) 95°C for 15 min (3) 95°C for 15 s (4) 55°C for 20 s (5) 72°C for 20 s + plate read (6) GOTO (3); 44 more times (7) 95°C for 1 min (8) Melting curve, 68 to 86°C, increment 0.2°C, 1 s + plate read End