Table 2.
Screening of mosquito pools collected in Greece. Review of the screening of the 340 mosquito pools (50 mosquitoes each) collected in Greece. All RT-qPCR positive and 6 RT-qPCR negative pools were tested further by microarray and direct sequencing. All other pools tested showed a Cq > 38 and Tm < 78°C.
| Mosquito pool | Cq | Tm (°C) | Microarray | DNA sequencing result |
|---|---|---|---|---|
| MPGr.01 | 37.3 | 81.4 | WNV.2 Austria | Nea Santa-Greece-2010 HQ537483 |
| MPGr.02 | 31.6 | 81.6 | WNV.2 Austria | Nea Santa-Greece-2010 HQ537483 |
| MPGr.03 | 21.5 | 81.7 | WNV.2 Austria | Nea Santa-Greece-2010 HQ537483 |
| MPGr.04 | 32.5 | 81.9 | WNV.2 Austria | Nea Santa-Greece-2010 (with 1 mutation) |
| MPGr.05 | 38.5 | 81.6 | WNV.2 Austria | Negative |
| MPGr.06 | 35.6 | 80,4 | ? | Marisma mosquito virus (93% identity) |
| MPGr.07 | 39.6 | 81.0 | Culex Flav | New Mosq Culex Flavivirus (78% identity) |
| MPGr.08 | 39.8 | 79.4 | Negative | Negative |
| MPGr.09 | 41.4 | 83.0 | Negative | Negative |
| MPGr.10 | 38.6 | 81.2 | Negative | Negative (residual sequence: Salmonella sp.) |
| MPGr.11 | 40.1 | 82.4 | Negative | Negative |
| MPGr.12 | 40.6 | 79.2 | Negative | Negative |
| MPGr.13 | 39.6 | 80.7 | Negative | Negative (residual sequence: Pseudomonas sp.) |