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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: J Heart Valve Dis. 2014 Jul;23(4):387–394.

Figure 5.

Figure 5

Synthesis of pyrophosphate from AV leaflets. A) Quantification of PPi released into the medium from AV leaflets incubated with or without 30 μM MLS38949 (an inhibitor of TNAP) as a function of time. * p <0.05 versus no MLS38949 at 5 h (n = 4). B) Autoradiogram of a representative thin-layer chromatogram after incubation of valve leaflets with [γ32P]ATP in 3 nM ATP with or without 30 μM MLS38949 or 300 μM β, γ-methylene ATP, an inhibitor of NPP1. All of the exogenous ATP was consumed in 5 min. Control leaflets were incubated without any inhibitors. C) Quantification of PPi production by AV tissue from ATP. * p <0.05 versus control.