Skip to main content
. 2017 May 23;2017:3631565. doi: 10.1155/2017/3631565

Figure 6.

Figure 6

Effects of FMF on APAP-induced cell death-related gene expression. (a) Total cellular protein from liver tissues was extracted, and protein levels of CYP2E1, cleaved caspase-3/9, caspase-3/9, Bax, Bcl2, p-JNK, JNK, p-ERK, and ERK were determined by western blot. GAPDH was used as an endogenous control. (b) Total RNA from liver tissues was isolated and reverse-transcribed into cDNA for quantitative real-time PCR analysis of Bax and Bcl2 mRNA levels. GAPDH was used as an endogenous control. Results are shown as mean ± SD (n = 3). ∗∗∗p < 0.001, ∗∗p < 0.01 compared with the control group; ###p < 0.001 compared with APAP-intoxicated group.