Table 6. Proposed recommendations for preclinical studies of nanoscale drug delivery systems with zebrafish models.

	Direct incubation	Direct injection
Treatment time point	6 hpf	48 hpf
Observation time point	48, 72, 96, 168 hpf	1, 24, 48, 72, 96, 120 hpi
Hatching/mortality rate observation time point	72 hpf
Heartbeat observation time point	48, 72, 96 hpf
Biodistribution observation	TEM analysis
Nanomaterials uptake quantitation	ICP-MS analysis

Bold: the time points for certain actions in preclinical studies

Abbreviation: hpi, h post injection.