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. 2017 Jun 7;37(3):BSR20160552. doi: 10.1042/BSR20160552

Figure 5. Rapamycin modulates cardiac signal transduction.

Figure 5

Rapamycin modulates cardiac signal transduction. (A) Representative images of Western blot analysis are shown to detect the expression of β-catenin and the nuclear accumulation of β-catenin. Lamin B1 is used as an internal control for nuclear, and GAPDH is used as an internal control for cytoplasm. Real-time PCR analysis of Wnt11 expression at indicated time points. Three independent experiments were performed. (B) Real-time PCR analysis of Hes1 in indicated time points. Three independent experiments are shown, each in triplicate; each bar represents mean ± S.D. of three samples (*P<0.05, **P<0.01 comapred with control). Representative images of Western blot analysis are shown to detect the activated NICD1 at indicated time points. Three independent experiments were performed. (C) Real-time PCR analysis of Fgf8, Fgf10, Noda1, Bmp2 and Bmp4 expression at indicated time points. Rapamycin: 20 nM rapamycin + AA treated from day 0 to day 12; control: DMSO (0.01%) + AA treated from day 0 to day 12. Three independent experiments are shown, each in triplicate; each bar represents mean ± S.D. from three samples (*P<0.05, **P<0.01 compared with control).