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. 2017 Jun 8;8:981. doi: 10.3389/fpls.2017.00981

FIGURE 5.

FIGURE 5

Expression pattern of RRP42 and subcellular localization of RRP42-GFP protein. (A) The ovule of stage 3-I. Bar = 5 μm. (B) Nine-day-old seedlings. Bar = 1 mm. (C) Six-week-old flowers. Bar = 1 mm. (D) Six-week-old rosette leaves 1–11. Bar = 5 mm. (E) Six-week-old cauline leaf 1 (left) and 2 (right). Bar = 5 mm. (F) Expression of RRP42 in various organs determined by qRT-PCR. Total RNA was isolated from roots, 7 to 8 rosette leaves, cauline leaves and flowers of 6-week-old WT plants and 9-day-old WT seedlings. Transcript levels were quantified by qRT-PCR against AT4G34270. The data are expressed as means ± SD of three independent biological determinations. (G) Intracellular distribution of RRP42-GFP and RFP-AHL22 proteins in living Arabidopsis protoplasts. Bars = 10 μm. (H) Intracellular distribution of RRP42-GFP proteins in root cells of 7-day-old stable Arabidopsis transformants. Bars = 20 μm.