Fig. 5.
Re-evaluation of chip-derived Streptavidin binders in complementary setups. a In vitro fluorescence polarization assays using constant amounts of 5’FAM labeled (chemically synthesized) peptides plus increasing concentrations of recombinant protein. Data are reported as mean from 3 independently performed measurements. Standard deviation was below 15% for all values. b Binding kinetics (association of pre-determined amounts 5’FAM labeled peptides to Streptavidin biosensors) were measured via biolayer-interferometry. Kd values as calculated from the BLItz Pro software are listed