Fig. 2.

Luminal filling and enhanced acini size observed upon myocardin-related transcription factor (MRTF) overexpression. Cells were grown on matrigel in medium containing 2% matrigel and 2% horse serum for 14 days as before. a Immunofluorenscence staining of acini formed by the indicated cell lines. Representative mid plane sections are shown for MRTF-A, MRTF-B, lamininV (red), phalloidin (red) and DNA (blue). Scale bar 20 μm. b Quantification of lumen formation detected from confocal images of 30 acini from three independent experiments. c Acini size measured from bright field images. Shown is the average mid plane area per cell line for at least 100 acini in three independent experiments. The box represents the interquartile range and the middle line represents the median; the whiskers extend to the 5th and 95th percentiles: *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test)