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. 2017 Jun 7;19:68. doi: 10.1186/s13058-017-0860-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — MCF10A acini formation is myocardin-related transcription factor (MRTF) dependent. Stably transduced cells harboring control or MRTF-A knockdown (shCtrl, shMRTF-A#1, shMRTF-A#2), recue of MRTF-A (sh#1 + MRTF-A, sh#2 + MRTF-A), rescue with MRTF-B (sh#1 + MRTF-B, sh#2 + MRTFB) or control rescue (shCtrl + GFP) were cultured in matrigel for 14 days as before. Immunofluorenscence staining of MRTF-A, MRTF-B, lamininV (red), phalloidin (red) and DNA (blue) from representative confocal mid plane sections are shown. Scale bar 20 μm