TABLE 5.

Age-adjusted intraclass correlation coefficients (ICC) and 95% confidence intervals for each biomarker across five categories of time between blood draws for 250 HIV-uninfected men, Multicenter AIDS Cohort Study (MACS), 1984 – 2009

	Interval 1		Interval 2		Interval 3		Interval 4		Interval 5
	0 – 2 years		2.1 – 4 years		4.1 – 8 years		8.1 – 15 years		> 15 years
	n = 223a		n = 355		n = 358		n = 183		n = 297
Biomarkers b	ICCc	95% CI	ICC	95% CI	ICC	95% CI	ICC	95% CI	ICC	95% CI
Chemokines
CCL11	0.90	(0.87 – 0.93)	0.89	(0.87 – 0.91)	0.82	(0.78 – 0.85)	0.77	(0.70 – 0.82)	0.81	(0.76 – 0.85)
CXCL8–chemo	0.57	(0.48 – 0.66)	0.49	(0.42 – 0.57)	0.40	(0.33 – 0.48)	0.30	(0.20 – 0.42)	0.24	(0.16 – 0.33)
CXCL10	0.78	(0.72 – 0.83)	0.77	(0.72 – 0.81)	0.76	(0.71 – 0.80)	0.69	(0.60 – 0.76)	0.68	(0.62 – 0.74)
CCL2	0.84	(0.80 – 0.88)	0.84	(0.81 – 0.87)	0.86	(0.82 – 0.88)	0.77	(0.71 – 0.83)	0.79	(0.74 – 0.83)
CCL13	0.91	(0.88 – 0.93)	0.89	(0.86 – 0.91)	0.84	(0.81 – 0.87)	0.76	(0.70 – 0.82)	0.83	(0.79 – 0.86)
CCL4	0.79	(0.73 – 0.84)	0.80	(0.76 – 0.84)	0.85	(0.82 – 0.88)	0.91	(0.88 – 0.93)	0.88	(0.84 – 0.90)
CCL17	0.89	(0.85 – 0.91)	0.88	(0.85 – 0.90)	0.75	(0.70 – 0.79)	0.66	(0.57 – 0.74)	0.73	(0.67 – 0.78)
CXCL13	0.84	(0.79 – 0.88)	0.78	(0.74 – 0.82)	0.90	(0.87 – 0.92)	0.52	(0.42 – 0.62)	0.48	(0.40 – 0.56)
Cytokines
IL-10	0.85	(0.81 – 0.88)	0.86	(0.82 – 0.89)	0.78	(0.74 – 0.82)	0.67	(0.58 – 0.74)	0.71	(0.64 – 0.76)
IL-12p70	0.84	(0.79 – 0.87)	0.86	(0.82 – 0.88)	0.78	(0.73 – 0.82)	0.70	(0.62 – 0.77)	0.73	(0.67 – 0.78)
IL-6	0.60	(0.51 – 0.68)	0.58	(0.51 – 0.65)	0.59	(0.52 – 0.65)	0.64	(0.55 – 0.72)	0.46	(0.38 – 0.55)
CXCL8-pro	0.59	(0.50 – 0.67)	0.50	(0.43 – 0.57)	0.39	(0.31 – 0.47)	0.24	(0.15 – 0.37)	0.23	(0.16 – 0.33)
TNF-α	0.54	(0.46 – 0.63)	0.56	(0.49 – 0.63)	0.46	(0.39 – 0.54)	0.42	(0.32 – 0.53)	0.49	(0.40 – 0.57)
BAFF	0.66	(0.58 – 0.73)	0.72	(0.66 – 0.77)	0.67	(0.61 – 0.73)	0.55	(0.45 – 0.65)	0.62	(0.54 – 0.68)
Soluble Cytokine Receptors
sCD14d	0.52	(0.43 – 0.61)	0.58	(0.51 – 0.65)	0.60	(0.53 – 0.66)	0.55	(0.45 – 0.65)	0.60	(0.52 – 0.67)
sCD27	0.78	(0.72 – 0.83)	0.81	(0.77 – 0.84)	0.80	(0.75 – 0.83)	0.74	(0.67 – 0.80)	0.72	(0.66 – 0.77)
sgp130	0.64	(0.56 – 0.72)	0.73	(0.67 – 0.77)	0.74	(0.68 –0.78)	0.60	(0.51 – 0.68)	0.60	(0.53 – 0.68)
sIL-2Rα	0.78	(0.72 – 0.83)	0.79	(0.74 – 0.83)	0.78	(0.73 – 0.82)	0.67	(0.58 – 0.74)	0.71	(0.65 – 0.76)
sTNF-R2	0.71	(0.64 – 0.77)	0.73	(0.67 – 0.78)	0.74	(0.69 – 0.79)	0.70	(0.62 – 0.77)	0.67	(0.60 – 0.73)

^aNumber of visit-pairs contributing to interval of time between blood draws.

^bUndetectable values were assigned a value equal to one-half the plate-specific lower limit of detection.

^cAge-adjusted. Age at visit was modeled continuously and centered at the median age across all 971 HIV-uninfected person-visits.

^dOne observation with an implausible value for sCD14 was excluded only from analyses of sCD14 (n = 970).

NOTE: CXCL8-pro denotes CXCL8 tested on the MSD proinflammatory cytokine kit; CXCL8-chemo denotes CXCL8 tested on the MSD chemokine kit