Table 2. PCR conditions for amplification of the RGR unit.
98 °C | 30 sec | 1 cycle |
98 °C | 10 sec | 30 cycles |
58 °C | 10 sec | |
72 °C | 15 sec | |
72 °C | 2 min | 1 cycle |
98 °C | 30 sec | 1 cycle |
98 °C | 10 sec | 30 cycles |
58 °C | 10 sec | |
72 °C | 15 sec | |
72 °C | 2 min | 1 cycle |