Table 3. In vitro DNA assembly .
| Final PCR product and the vector | 0.03-0.2 pmols, X μl |
| Recommended DNA ratio | Vector:Insert = 1:2 |
| NEB HiFi DNA assembly master mix (Gibson Assembly® Cloning Kit) | 10 µl |
| Water | 10-X μl |
| Total Volume | 20 µl |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.