Skip to main content
. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Mol Microbiol. 2016 Sep 26;102(5):865–881. doi: 10.1111/mmi.13496

Figure 1.

Figure 1

Soluble cell wall components liberated by LtgA and LtgD separated by HPLC. Reactions were performed by incubating 500 μg of purified gonococcal sacculi with purified LtgA, LtgD, or corresponding active site mutants. Soluble products were separated by reversed-phase HPLC using an ACN gradient. Sacculi from strains lacking O-acetylation and wild-type strains were digested. Peaks were identified by LC-MS of similarly digested sacculi. G, N-acetylglucosamine; aM, 1,6-anhydro-N-acetylmuramic acid; numbers represent the number of peptide stem amino acids (L-Ala, D-Glu, meso-DAP, D-Ala, D-Ala).