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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Mol Microbiol. 2016 Sep 26;102(5):865–881. doi: 10.1111/mmi.13496

Figure 2.

Figure 2

Liberated PG monomers from sequential digestion of sacculi with LtgA and LtgD. Soluble PG fragments from whole sacculi digests with LtgA or LtgD followed by boiling and a subsequent digest with LtgA or LtgD were separated by HPLC. The abundance of PG monomers (tripeptide (GaM-3) and tetrapeptide (GaM-4)) was determined by calculating the peak area (AUP) from three independent experiments. Horizontal bars indicate significance of P < 0.05, and (*) indicates P < 0.01 by Student’s t-test. Error bars represent standard deviation.