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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Mol Microbiol. 2016 Sep 26;102(5):865–881. doi: 10.1111/mmi.13496

Figure 3.

Figure 3

Digestion of a synthetic tetrasaccharide dipeptide. (A) A tetrasaccharide dipeptide was incubated with the indicated LT, and the resulting products were separated by reversed-phase HPLC using an ACN gradient. Incubation with LtgA and LtgD yielded two peaks corresponding to (i) reducing pentapeptide monomer and (ii) anhydro pentapeptide monomer. Digestion with LtgC and the no enzyme control yielded a single peak (iii) corresponding to undigested tetrasaccharide dipeptide. (B) Chemical structure of synthetic compound iii and observed digestion products with exact masses confirmed by mass spectrometry. The arrow indicates the site of LtgA and LtgD activity that results in the formation of a 1,6-anhydro bond indicative of LT activity.