Table 1.

LC-MS of soluble fragments released by digests of sacculi with LtgA or LtgD. Parentheses indicate detector counts were less than 0.5% of the total run. G, N-acetylglucosamine; aM, 1,6-anhydro-N-acetlymuramic acid; numbers represent the number of peptide stem amino acids. GaM-4(Gly) denotes a glycine in the fourth position.

				LtgA		LtgD
PG Fragment	Calculated m/z	Calculated Exact Mass	Ion	Observed m/z	Observed Mass	Observed m/z	Observed Mass
GaM	479.18	478.18	[M+H]	479.19	478.18	(479.19)	(478.18)
GaM-3	851.34	850.34	[M+H]	851.35	850.34	851.35	850.34
GaM-3-1	512.21	1022.43	[M+2H]2+	(512.22)	(1022.43)	(512.23)	(1022.44)
GaM-4(Gly)	908.37	907.37	[M+H]	908.37	907.37	(454.69)	(907.37)
GaM-3-3	612.25	1222.51	[M+2H]2+	612.26	1222.51	(612.27)	(1222.52)
GaM-4	922.38	921.38	[M+H]	922.39	921.39	922.39	921.38
GaM-4-3	647.77	1293.55	[M+2H]2+	647.78	1293.55	(647.78)	(1293.55)
GaM-5	993.42	992.42	[M+H]	993.43	992.42	993.43	992.42
GaM-2	679.26	678.26	[M+H]	679.27	678.26	679.27	678.26
GaM-4-2	583.25	1164.50	[M+2H]2+	(583.26)	(1164.51)	(583.26)	(1164.51)
GaM-4-4	683.29	1364.58	[M+2H]2+	683.30	1364.59	(683.30)	(1364.59)
GaM-4-4-4	603.60	1807.79	[M+3H]3+	(603.60)	(1807.79)	-	-
GaM-3-3-GaM	842.34	1682.68	[M+2H]2+	(842.35)	(1682.68)	(842.35)	(1682.68)
GaM-4-3-3-GaM	1064.44	2125.88	[M+3H]2+	(1064.45)	(2125.88)	-	-
GaM-4-4-3-GaM	733.64	2196.92	[M+4H]3+	(733.65)	(2196.92)	-	-
GaM-4-4-4-GaM	757.32	2267.95	[M+4H]3+	(757.33)	(2267.96)	-	-
GaM-4-3-GaM	877.86	1753.72	[M+2H]2+	877.87	1753.72	(877.87)	(1753.72)
GaM-4-4-GaM	913.38	1824.75	[M+2H]2+	913.38	1824.75	(913.39)	(1824.76)