Figure 6. Heterozygous animals with one edited disrupted allele of F28F8.5 and one WT allele.

Heterozygous hermaphrodites carrying one edited allele of F28F8.5 and one WT had grossly normal appearance and could be recognized by rol phenotype, presence of embryos, weak mostly cytoplasmic GFP fluorescence and absence of nuclear localization of GFP fluorescence. Panels A (Nomarski optics) and B (GFP fluorescence) show an L3 larva with weak fluorescence (panel B, arrowhead points at the gonad and arrows point at the head and pharynx). Inlets show head area at higher magnification (rotated 90° clockwise). Panels C and D show an adult hermaphrodite animal (C in Nomarski optics and D in GFP fluorescence) with weak cytoplasmic fluorescence in most cells. The arrowhead in panel D points at the nucleus of an enterocyte in focal plane that is devoid of GFP fluorescence. Arrows indicate two embryos with GFP fluorescence accumulated in nuclei which is most likely the result of spontaneous SEC self-excision. Panels E and F show an adult hermaphrodite in Nomarski optics (panel E) and GFP fluorescence (panel F). Arrows indicate the head area with diffuse intracellular fluorescence visible in panel F. Arrowheads point at two nuclei of enterocytes in focal plane that are also devoid of fluorescence. In contrast to the animal shown in panels C and D, the animal shown in the panel E and F contains embryos that have mostly diffuse cytoplasmic expression of GFP. Bars represent 50 μm.