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Rnd3 gradually disappears from blebbing membrane at the onset of the retraction phase. Membrane blebbing of a DLD1 cell, a human colon cancer cell, transfected with Lifeact–RFP and GFP-tagged Rnd3. Rnd3 was recruited to expanding membrane blebs; however, the membrane localization of Rnd3 is gradually lost during the retraction of membrane blebs. (Scale bar, 2 μm.)
