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. 2016 Jun 29;8(2):114–121. doi: 10.1080/21541248.2016.1205334

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Figure 3. — Arg188 promotes a more active Rho phenotype than Ser188. OVCA429 cells transfected with the indicated Myc-Rho expression plasmid were immunostained for the c-Myc epitope and qualitatively examined for actin-driven cell rounding. Myc-RhoA and Myc-RhoC-R188S expressing cells displayed a mixture of both normal and rounded phenotypes independent of fluorescence intensity (arrows). On the other hand, Myc-RhoA-S188R and Myc-RhoC expressing cells were nearly exclusively the rounded cellular phenotype (arrow), suggesting greater ho-driven actin contractility activity when Arg188 is present. Inset panels provide a magnified view of arrow directed fields. ar = 205 μm.