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. 2016 Jun 29;8(2):114–121. doi: 10.1080/21541248.2016.1205334

Figure 6.

Figure 6.

(A) To examine whether presence of a serine or loss of the arginine has the greatest impact on Myc-RhoC membrane association, membrane association of Myc-RhoC-R188S was compared to Myc-RhoC-R188A. Western blot analysis of membrane fractionations revealed both mutations (R188S and 188A) negatively impacted membrane stability to the same extent (n = 6,6 U = 14 z = 0.56 p = 0.5755 for Myc-RhoC-R188S vs. Myc-RhoC-R188A). By densitometric analysis, P100/S100 ratio for Myc-RhoC-R188S was 0.29 ± 0.27 and Myc-RhoC-R188A 0.20 ± 0.15 when compared to Myc-RhoC. (B) At the same time, Myc-RhoA-S188R was more membrane associated than Myc-RhoA-S188A. P100/S100 ratio was 2.05 ± 0.78 higher for Myc-RhoA-S188R when compared to Myc-RhoA-S188A (n = 9,9 U = 78 z = −3.31 p = 0.0009).