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. 2005 Feb 1;115(2):291–301. doi: 10.1172/JCI22681

Figure 6.

Figure 6

Lack of effect of Munc18c deficiency on proximal insulin signaling in adipocytes. Munc18c+/+, Munc18c+/–, or Munc18c–/– adipocytes were deprived of serum for 2 hours and then stimulated with insulin (1 or 100 nM) for 5 minutes. For detection of tyrosine-phosphorylated forms of IRS (pY-IRS) and the β subunit of the insulin receptor (pY-IRβ), cell lysates were subjected to immunoprecipitation and subsequent immunoblot analysis with mAb PY20 to phosphotyrosine (upper panel). For detection of serine-phosphorylated Akt, cell lysates were subjected to immunoblot analysis with antibodies specific for Akt phosphorylated on serine 473 (lower panel).