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. 2017 Jun;119:48–61. doi: 10.1016/j.neuropharm.2017.01.023

Fig. 4.

Fig. 4

Expression of mutant 5-HT3 receptors. Three of the mutant receptors did not bind either [3H]tropisetron or [3H]granisetron (W90C, E129C, W183C). To confirm that this could be attributed to altered binding rather than changes in expression, these mutants were probed by Western blot using a 5-HT3 receptor-specific antibody. Expression of the mutants and wild type receptors were comparable, and there was no detection in untransfected cells. In each lane 105 cell equivalents were loaded and an antibody against the cleavage and polyadenylation specificity factor (CPSF) included as an internal control. Note that whole-cell homogenates were used for both radioligand binding and Western blot to enable monitoring of the same populations of both intracellular and cell-surface receptors.