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Relative quantification of overexpressed wild type and variant aromatase in HEK293 microsomes by Western blotting. (A) Lane 1, wild type aromatase; lane 2, aromataseThr201Met; lane 3, aromataseArg264Cys. Aromatase was detected using a monoclonal anti‐aromatase antibody (Abcam). (B) Ponceau total protein staining for aromatase normalization. Lanes correspond to the same three lanes described in panel A.
