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. 2017 Apr 27;5(3):e00314. doi: 10.1002/prp2.314

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© 2017 The Authors. Pharmacology Research & Perspectives published by John Wiley & Sons Ltd, British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Relative quantification of overexpressed CYP450s in HEK293 microsomes. Panel (A), Detection of V5‐tagged CYP450s by Western blotting. Panel (B), Ponceau total protein staining for CYP450 normalization. Lane 1, CYP2C19; lane 2, CYP2C19^Glu92Asp; lane 3, CYP2C19^Ile331Val; lane 4, CYP2D6; lane 5, CYP2D6^{Arg296Cys, Ser486Thr}; lane 6, CYP2D6^{Pro34Ser, Ser486Thr}; lane 7, CYP2D6^{Thr107Ile, Arg296Cys, Ser486Thr}; lane 8, CYP3A4; lane 9, CYP3A4^Arg162Gln; lane 10, CYP3A5.