Figure 6.

Concentration‐dependent Reversal of PGE₂ effects on TNFα production by EP4 antagonists Pooled human whole blood samples (triplicate) from normal volunteers were pre‐treated with various concentrations of compound 1(Panel A), compound 2(Panel B) or reference EP4 inhibitor (CJ‐023,423) for 30 min and TNFα production determined as described in Materials and Methods. The figure is a representative of results from experiments of n = 8 for compound‐1, n = 12 for compound‐2 and n = 79 for CJ‐023,423. Panel A and B: ▾LPS control;● LPS+PGE₂;■ compound + LPS + PGE₂; ▴ CJ‐023,423 + LPS + PGE_2.