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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Immunol Res. 2017 Jun;65(3):739–749. doi: 10.1007/s12026-017-8913-3

Figure 3. Blockade of Notch signaling pathway inhibits Hes1 and induces HMGB1/TLR4 and NLRP3 activation in APAP-induced liver injury.

Figure 3

(A) Western-assisted expression of Hes1, HMGB1, TLR4, NF-κB, and NLRP3 in APAP-challenged livers with DAPT or DMSO precondition. β-actin served as an internal control. Data representative of three experiments. (B) Density ratios of Hes1, HMGB1, TLR4, NF-κB, and NLRP3. *p<0.05, **p<0.01. (C) Quantitative RT-PCR-assisted detection of IL-1β, TNF-α, and IL-6 in mouse livers. Each column represents the mean±SD (n=3–4 samples/group). *p<0.05.