Blocking Notch signaling by γ-secretase inhibitor DAPT suppresses the cleavage of Notch1 to NICD, which translocates into the nucleus to activate Notch target gene Hes1. Inhibition of Hes1 promotes HMGB1-TLR4 axis, which in turn triggers NLRP3 activation, leading to augmented caspase-1-mediated IL-1β secretion in APAP-induced liver inflammation. In addition, inhibition of Hes1 diminishes Stat3 and Akt phosphorylation, resulting in increased cell apoptosis/necrosis and reduced cell survival in APAP-challenged livers.