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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: J Struct Biol. 2016 Jun 8;195(2):190–198. doi: 10.1016/j.jsb.2016.06.001

Table 2.

Timing interval between spotting and observation that the substrate appears dry for nanowire grids spotted with a variety of sample volumes after varying intervals of dwell time inside an environment set at 80% humidity.

Grid dwell
time in 80%
humidity
chamber
before
spotting
(minutes)		 10 drops
(4nL);
time to wick
dry (s); rate of
volume
removal (nL/s)		 20 drops
(8nL);
time to wick
dry (nL/s); rate
of volume
removal (nL/s)		 30 drops
(12nL);
time to wick
dry (nL/s); rate
of volume
removal (nL/s)		 40 drops
(16nL);
time to wick
dry (nL/s); rate
of volume
removal (nL/s)
0 0.20s; 20nL/s 0.32s; 25nL/s 0.44s; 27nL/s 1.24s; 13nL/s
1 0.24s; 17nL/s 0.40s; 20nL/s 0.48s; 25nL/s 1.0s; 16nL/s
2 0.32; 12nL/s 0.44s; 18nL/s 0.60s; 20nL/s 0.96s; 17nL/s