Reagents	Manufacturer	Brand	Catalog number	Preparation	Aliquots	Storage	Stock concentration	Working concentration	Dilution
1	Hydrochloric acid (HCl) 1 N (sterile filtered)	Carl Roth		K025.1	1 L		RT		0.05 N	1 ml HCl 1 N + 19 ml H2O, sterile
2	Dulbecco’s PBS (DPBS)	Biowest		L0615-500	500 ml		4 °C	1x	1x	undiluted
3	70% EtOH (denatured)	Th. Geyer		2270			RT
4	Isoflurane	Baxter		KDG9623			RT
5	Collagenase/dispase (sterile filtered)	Roche Diagnostics		11097113001	500 mg/5 ml H2O, sterile	200 µl	-20 °C	100 mg/ml	1 mg/ml	100 µl collagenase/dispase in 10 ml medium
6	DNase I	Roche Diagnostics		11284932001	100 mg/10 ml H2O, sterile	100 µl	-20 °C	10 mg/ml	4 µg/ml	40 µl DNase I in 10 ml medium
7	Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK)	Sigma-Aldrich		90182	14.7 mg/10 ml H2O, sterile	200 µl	-20 °C	1.47 mg/ml diluted to 14.7 µg/ml	0.147 µg/ml	100 µl TLCK in 10 ml medium
8	Puromycin	Sigma-Aldrich		P8833	2.5 mg/10 ml H2O, sterile	500 µl	-20 °C	0.25 mg/ml	8 µg/ml	32 µl puromycin in 1 ml medium
9	Trypsin-EDTA 0.25%	Thermo Fisher Scientific	GibcoTM	25200-056	100 ml		-20 °C	1x	1x	undiluted
10	4% paraformaldehyde	Merck Millipore		1040051000	4% paraformaldehyde in DPBS		-20 °C
11	CD31	BD	BD Pharmingen	557355						1:500