1 |
Hydrochloric acid (HCl) 1 N (sterile filtered) |
Carl Roth |
|
K025.1 |
1 L |
|
RT |
|
0.05 N |
1 ml HCl 1 N + 19 ml H2O, sterile
|
2 |
Dulbecco’s PBS (DPBS) |
Biowest |
|
L0615-500 |
500 ml |
|
4 °C |
1x |
1x |
undiluted |
3 |
70% EtOH (denatured) |
Th. Geyer |
|
2270 |
|
|
RT |
|
|
|
4 |
Isoflurane |
Baxter |
|
KDG9623 |
|
|
RT |
|
|
|
5 |
Collagenase/dispase (sterile filtered) |
Roche Diagnostics |
|
11097113001 |
500 mg/5 ml H2O, sterile
|
200 µl |
-20 °C |
100 mg/ml |
1 mg/ml |
100 µl collagenase/dispase in 10 ml medium |
6 |
DNase I |
Roche Diagnostics |
|
11284932001 |
100 mg/10 ml H2O, sterile
|
100 µl |
-20 °C |
10 mg/ml |
4 µg/ml |
40 µl DNase I in 10 ml medium |
7 |
Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK)
|
Sigma-Aldrich |
|
90182 |
14.7 mg/10 ml H2O, sterile
|
200 µl |
-20 °C |
1.47 mg/ml diluted to 14.7 µg/ml |
0.147 µg/ml |
100 µl TLCK in 10 ml medium |
8 |
Puromycin |
Sigma-Aldrich |
|
P8833 |
2.5 mg/10 ml H2O, sterile
|
500 µl |
-20 °C |
0.25 mg/ml |
8 µg/ml |
32 µl puromycin in 1 ml medium |
9 |
Trypsin-EDTA 0.25% |
Thermo Fisher Scientific |
GibcoTM
|
25200-056 |
100 ml |
|
-20 °C |
1x |
1x |
undiluted |
10 |
4% paraformaldehyde |
Merck Millipore |
|
1040051000 |
4% paraformaldehyde in DPBS |
|
-20 °C |
|
|
|
11 |
CD31 |
BD |
BD Pharmingen |
557355 |
|
|
|
|
|
1:500 |