Table 1.
Cell-Based Assay Using the Human Cell Line TOM-1 to Compare Different Conditions for Obtaining High Reproducibility and Homogeneity of Cell Counts with an Automated Flow cytometry Platform.a
| Condition | Coefficient of Variation (%) | Differences, Max-Min (%) | Yield (%) |
|---|---|---|---|
| PharmaFlow platform | 3.77 | 14.69 | 81.6 |
| Other platforms | |||
| No shaking/no mixing/ no temperature control | 21.81 | 54.53 | 58.4 |
| Mixing via aspiration | 17.33 | 53.30 | 54.4 |
a
Sample acquisition conditions for the PharmaFlow platform were shaking at 1000 rpm with temperature controlled at 20 °C. An initial step of 5 min of shaking is performed for the three conditions.