Fig. 6.

NFkB activation in floating cells. (A) NS stably expressing NFkB-Gluc and SV40-Vluc were differentiated in serum and FC were collected from them. The normalized Gluc/Vluc level, a marker for NFkB activity, was analyzed in an aliquot of conditioned medium from NS and FC; **P < .01 FC vs NS (mean±SD, n = 8). (B) Cell lysates from NS and FC of MGG23 and BT01 were analyzed by western blotting for phosphorylated form and total p65 (ser536) as well as β-actin ratio of p-p65 over p65 is presented. (C) NS and FC mRNA from MGG23 and BT01 were analyzed by qRT-PCR for different NFκB target genes. Fold change relative to expression in NS are presented; ** P < .01 FC vs NS (mean±SD, n = 8).