Table 1.
Name | Primers (5 to 3) | PCR conditions | ||||
---|---|---|---|---|---|---|
Forward | Reverse | Annealing Temprature C | Annealing | Cycles n | Size bp | |
UI138 | ATGGACGATCTGCCGCTGAA1 | TCACGTGTATTCTTGATGAT1 | 52 | 60 sec | 35 | 510 |
IE1 | GCTTACCACTGGCACGACACCT | TACTCCCCGTACAGCTCGCAAC | 69 | 60 sec | 40 | 89 |
AGCCTTCCCTAAGACCACCAAT | CATAGCAGCACAGCACCCGACA | 57 | 60 sec | 40 | 260 |