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. 2017;18(3):593–597. doi: 10.22034/APJCP.2017.18.3.593

Table 1.

Primers and Amplification Conditions Used for Detection of UL138 and IE1

Name Primers (5 to 3) PCR conditions
Forward Reverse Annealing Temprature C Annealing Cycles n Size bp
UI138 ATGGACGATCTGCCGCTGAA1 TCACGTGTATTCTTGATGAT1 52 60 sec 35 510
IE1 GCTTACCACTGGCACGACACCT TACTCCCCGTACAGCTCGCAAC 69 60 sec 40 89
AGCCTTCCCTAAGACCACCAAT CATAGCAGCACAGCACCCGACA 57 60 sec 40 260