Table 1.

The Expression of Antigen in Tumor Tissue by the immunohistochemical Observations

Antigen expression	Score	Experimental group
		Control (n=8)	Gemcitabine (20 mg/kg) (n=8)	GM (100mg/kg) (n=8)
		% (n)	%(n)	% (n)
Cytokeratin 19 antigen (CK 19)	0	0 (0.0)	0 (0.0)	0 (0.0)
	1	0 (0.0)	4 (50.0)	3 (37.5)
	2	6 (75)	2 (25.0)	2 (25)
	3	1 (12.5)	1 (12.5)	2 (25)
	4	1 (12.5)	1 (12.5)	1 (12.5)
Proliferating cell nuclear antigen (PCNA)	0	0 (0.0)	5 (62.5)*	4 (50)*
	1	0 (0.0)	3 (37.5)	3 (37.5)
	2	1 (12.5)	0 (0.0)	1 (12.5)
	3	4 (12.5)	0 (0.0)	0 (0.0)
	4	3 (37.5)	0 (0.0)	0 (0.0)

Score: 0, no or a few positive area (< 10%); 1, positive area (< 25%); 2, no or a few positive area (< 50%); 3, no or a few positive area (< 75%); 4, no or a few positive area (> 75%); Significantly different compared with untreated control at the same time point

^*(p< 0.05)