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. 2017 Jun 8;12(6):e0178494. doi: 10.1371/journal.pone.0178494

Table 2. Effects of irrigation and nitrogen application on grain yield and protein content in winter wheat in 2012/2013, 2013/2014 and 2014/2015, and interactions between irrigation and nitrogen application; summary of F significance from analysis of variance of the effects of main factors and interactions.

Treatment Yield (t ha-1) Protein (%)
2012/2013 2013/2014 2014/2015 2012/2013 2013/2014 2014/2015
I0 4.99b 6.16b 6.92c 14.76 15.33 14.33
I1 5.37b 7.19a 7.81b 13.34 14.91 14.33
I2 6.45a 7.74a 8.00a 14.22 14.89 14.35
F-test ** ** ** ns ns ns
N0 4.58b 4.47b 4.80b 11.57c 14.20b 10.86c
N180 5.73a 7.84a 8.41a 14.32b 15.22a 15.17b
N240 6.03a 7.76a 8.55a 14.91ab 15.40a 15.54a
N300 6.08a 8.05a 8.54a 15.62a 15.34a 15.77a
F-test ** ** ** ** ** **
I0×N0 3.71b 3.57b 4.32b 12.57b 14.37b 11.50b
I0×N180 4.94ab 6.67a 7.73a 15.33a 15.53a 15.10a
I0×N240 5.62a 7.34a 7.81a 15.37a 15.83a 15.10a
I0×N300 5.69a 7.07a 7.81a 15.77a 15.57a 15.60a
F-test * ** ** ** ** **
I1×N0 4.46b 4.60b 5.11b 11.50c 13.83b 10.67c
I1×N180 5.58a 8.39a 8.51a 12.40b 15.13a 15.03b
I1×N240 5.76a 7.63a 8.73a 13.93ab 15.30a 15.67a
I1×N300 5.70a 8.13a 8.87a 15.53a 15.37a 15.93a
F-test ** ** ** ** ** **
I2×N0 5.56b 5.25b 4.98b 10.63b 14.40b 10.40c
I2×N180 6.65a 8.48a 8.99a 15.23a 15.00a 15.37b
I2×N240 6.72a 8.31a 9.11a 15.43a 15.07a 15.87a
I2×N300 6.86a 8.93a 8.93a 15.57a 15.10a 15.77a
F-test * ** ** ** ** **
Grand mean 5.60 7.03 7.57 14.11 15.04 14.33
I×N (F-test) ns ns ns ** ns **
CV (%) 4.32 6.73 0.57 9.02 0.74 0.55

Note: ns, not significant at P < 0.05;

* Significant at P < 0.05;

** Significant at P < 0.01; Data in the same column with different letters indicate a significant difference at P < 0.05.