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. 2005 Mar 1;115(3):733–738. doi: 10.1172/JCI200523001

Figure 5.

Figure 5

TXNIP siRNA inhibited TNF activation of p38 and JNK but not ERK1/2 in HUVECs. After HUVECs were transfected with either control or TXNIP siRNA, TNF (10 ng/ml) was added for 15 minutes. MAPK activation was determined by immunoblotting using phospho-specific (p-) antibody. Equal loading was confirmed with total MAPK antibodies. Representative blots from 3 independent experiments are shown. Quantitation of the ratio of phospho-MAPK in lysates from cells treated with control or TXNIP siRNA to phospho-MAPK in lysates from cells not treated with siRNA is shown below each panel (average of 3 determinations).