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TNF-induced VCAM1 expression was decreased in TXNIP-deficient mouse aorta. After aortae from HcB-19 and control C3H mice were treated with TNF (15 ng/ml, 6 hours), vessel protein was harvested. Expression of VCAM1 and TXNIP was determined by immunoblotting in 2 aortic samples from 2 animals of each strain. Equal protein loading was confirmed with actin antibody.
