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. 2017 Jun 8;12(6):e0178601. doi: 10.1371/journal.pone.0178601

Fig 3.

Fig 3

A) Coomassie stained gel used for mass spectrometry analysis. High MW bands were readily apparent (arrows) in the pooled low-density membrane pellet sample (lane A). After processing the low-density membrane pellet by SEC, high MW bands disappeared (lane B). B) Western blot for CD63. Two bands of approximately 50 kDa were seen with the SEC vesicle fraction (fraction 6–10) (lane B). The low-density membrane pellet (lane A) and SEC fraction 11–30 (lane C) samples showed no bands with the anti-CD63 antibody.