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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: J Neurovirol. 2016 Sep 28;23(1):152–157. doi: 10.1007/s13365-016-0480-1

Fig. 3.

Fig. 3

Accumulation of VZV DNA in dissociated trigeminal ganglia (TG) in tissue culture with or without PI3-kinase inhibitor (LY294003) and anti-NGF antibody. Human TG were mechanically dissociated and incubated in six-well glass plates before addition of PI3-kinase inhibitor (LY294003) or anti-NGF antibody at day 0. At days 0 and 5, DNA was extracted and VZV DNA was quantified by PCR. The VZV DNA fold change was calculated by ΔCt analysis and normalized to 1 at day 0. Fold change in cultures treated with PI3-kinase inhibitor (LY294003) or anti-NGF antibody at day 5 was calculated as 2−(Ctday 5 − Ctday0). VZV DNA abundance did not differ (p = 0.87) between untreated control and LY294003-treated TG cultures, but was significantly increased (p = 0.001) in TG cultures treated with anti-NGF antibody