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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: Clin Sci (Lond). 2015 Jun 22;129(8):757–767. doi: 10.1042/CS20150008

Figure 1. Time-dependent increase of densities of SP and microglia in the SN.

Figure 1

(A) SP and microglia were stained with anti-SP and anti-Iba-1 antibody, respectively, in the SN at postnatal day 1 (P1), 7 (P7), 14 (P14) and 30 (P30) in C57BL/6 mice. (B) The densities of SP in the SN and adjacent area (as shown in A) were quantified by analyzing pixel density of SP staining using ImageJ. (C) Microglial density in the SN and adjacent area (as shown in A) was quantified by counting Iba-1-immunoreactive cells. A gradual increase of densities of SP and microglia in the SN was observed from P1 to P30. (D) Compared with adjacent area, fold increases of SP and microglial densities in the SN during postnatal development were calculated. (E) A positive correlation between increased expression of SP and microglial densities in the SN from P1 to P30 was observed. Results of microglial density are expressed as mean ± SEM, while data of SP density are expressed as a percentage of P1 adjacent area (mean ± SEM). *p< 0.05, **p< 0.01. n = 4–8 in each group at each time point with total 18. Bar = 200 μm.