Figure 2. Lack of endogenous SP and NK1R decreases microglial density in the SN.
Microglia were stained with anti-Iba-1 antibody in the SN and its adjacent area of post-natal day 1 (P1), 7 (P7), 14 (P14) and 30 (P30) WT, TAC1−/− and NK1R−/− mice. (A–C) The density of microglia in the SN (A and C) and its adjacent area (B) was quantified by counting Iba-1-immunoreactive cells. Results are expressed as mean ± SEM. *p< 0.05, **p< 0.01. n = 4–8 in each group at each time point with total 35, 18 and 18 for WT, TAC1−/− and NK1R−/− mice, respectively.
