Figure 3. SP fails to affect microglial proliferative capacity.

(A) Proliferating microglia were double stained with anti-Iba-1 and anti-PCNA antibodies in the SN of P7 WT and TAC1^−/− mice. Representative images in different magnifications were shown. (B) Iba-1⁺PCNA⁺ and Iba-1⁺ microglia were counted and the proliferative rate was calculated. n = 6 and 8 for WT and TAC1^−/− mice, respectively. (C) Mixed glia cultures were pre-incubated with BrdU (20 μmol/l) for 30 mins and followed by SP (10⁻⁸ M) or vehicle treatment for additional 72h. Proliferated microglia were recognized by double BrdU and Iba-1 immunoreactive cells. (D) The percentage of Iba-1⁺BrdU⁺ cells was calculated with respect to the total number of Iba-1⁺ cells. Results are expressed as mean ± SEM. n = 3. Bar = 50 μm.