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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: Clin Sci (Lond). 2015 Jun 22;129(8):757–767. doi: 10.1042/CS20150008

Figure 5. NOX2-generated superoxide is a critical mediator in SP-induced microglial chemotaxis.

Figure 5

(A) Mixed-glia cultures (containing 80% astroglia and 20% microglia) were treated with 10−7 or 10−8 M SP. Superoxide production was measured using SOD-inhibitable reduction of tetrazolium salt WST-1. (B) SP failed to produce superoxide in mix-glia cultures prepared from gp91phox−/− mice. Data are expressed as the percentage of controls (mean ± SEM). (C) Chemotaxis assay was performed by pre-treating microglia with NOX2 inhibitor DPI (10−13 M) for 30 mins and followed by SP (10−8 M) stimulation. (D) Purified microglia were prepared from WT and gp91phox−/− mice. The chemotaxis capacity of SP on WT and gp91phox−/− microglia was assessed. Results are expressed as the migration index (mean ± SEM). *p< 0.05, **p< 0.01. n = 4–5.